Figure 2: Agarose gel electrophoresis (2%) of kDNA(RV1-RV2)-PCR (145 bp) from positive samples of patients with visceral leishmaniasis (VL). (50) 50 bp DNA ladder. (R) Negative control of reagents room. (E) Negative control of extraction room. Lanes 1, 2, 3 and 4 samples of patients with VL. Lanes 5 and 7-samples of patients with VL but negative in kDNA (RV1-RV2). (PC) L. (L.) infantum positive control.