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Figure 2: Representative photograph of 1.5% agarose gel showing the amplified product of 450 bp region (ASA.S1) of the 18S rRNA gene of Acanthamoeba spp. Lane A: positive control (A. castellanii ATCC 50492); Lane B: negative control (distilled water); Lanes 1-14: positive isolates; Lane M: DNA ladder (100 bp). |
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