Asian Pacific Journal of Tropical Medicine

ORIGINAL ARTICLE
Year
: 2022  |  Volume : 15  |  Issue : 7  |  Page : 314--321

Mosquito larva distribution and natural Wolbachia infection in campus areas of Nakhon Ratchasima, Thailand


Thunyarat Surasiang1, Sirilak Chumkiew2, Pongsakorn Martviset3, Pathanin Chantree3, Mantana Jamklang4 
1 Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhon Pathom, Thailand
2 School of Biology, Institute of Science, Suranaree University of Technology, Thailand
3 Research Unit in Nutraceuticals and Food Safety; Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathumthani, Thailand
4 School of Preclinical Sciences, Institute of Science, Suranaree University of Technology, Thailand

Correspondence Address:
Mantana Jamklang
School of Preclinical Sciences, Institute of Science, Suranaree University of Technology
Thailand

Objective: To determine the prevalence of mosquito larvae in campus areas and the infection rate of endosymbiotic bacteria, Wolbachia in mosquito larvae. Method: The mosquito larvae samples were collected in residential areas and academic buildings of Suranaree University of Technology located in Northeastern Thailand during 2017-2018. Mosquito species identification was performed using GLOBE mosquito protocols and Rattanarithikul & Panthusiri’s keys. The gene encoding for the surface protein of Wolbachia was amplified by PCR and confirmed by DNA sequencing. Results: Armigeres sp. is the highest proportion of mosquito larvae followed by Culex spp., Aedes albopictus, Aedes aegypti, and Toxorynchites spp., respectively. Aedes aegypti have breeding sites mostly in the containers found indoors, whereas the main breeding sites of Aedes albopictus were found in both outdoors and indoors. The House Index and Breteau Index for Aedes spp. was more than 5% and 20%, respectively, in both areas, indicating that these areas are dengue sensitive. The highest proportion of Wolbachia infection was found in the larvae of Culex spp. (86.21%), followed by Aedes albopictus (69.23%) and rarely detected in Aedes aegypti (9.09%). Conclusion: The present study reported the first natural infection of Wolbachia in mosquito larvae in Thailand. Our result suggested that the mosquito species containing higher proportion of Wolbachia are less likely to be vectors for dengue. Therefore, Wolbachia transfection in mosquito larvae could be applied as a biocontrol for dengue and other mosquito-borne disease prevention.


How to cite this article:
Surasiang T, Chumkiew S, Martviset P, Chantree P, Jamklang M. Mosquito larva distribution and natural Wolbachia infection in campus areas of Nakhon Ratchasima, Thailand.Asian Pac J Trop Med 2022;15:314-321


How to cite this URL:
Surasiang T, Chumkiew S, Martviset P, Chantree P, Jamklang M. Mosquito larva distribution and natural Wolbachia infection in campus areas of Nakhon Ratchasima, Thailand. Asian Pac J Trop Med [serial online] 2022 [cited 2022 Aug 12 ];15:314-321
Available from: https://www.apjtm.org/article.asp?issn=1995-7645;year=2022;volume=15;issue=7;spage=314;epage=321;aulast=Surasiang;type=0