Asian Pacific Journal of Tropical Medicine

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 14  |  Issue : 10  |  Page : 451--455

Prevalence of non-tuberculosis mycobacteria among samples deposited from the National Tuberculous Reference Laboratory of Iran (2011-2018)


Saman Ayoubi1, Parissa Farnia1, Poopak Farnia2, Jafar Aghajani1, Jalaledin Ghanavi1, Ali Akbar Velayati1 
1 Mycobacteriology Research Center (MRC), National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Department of Biotechnology, School of Advanced Technology in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Correspondence Address:
Saman Ayoubi
Mycobacteriology Research Center (MRC), National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran
Iran

Objective: To investigate the prevalence of non-tuberculosis mycobacteria (NTM) among the samples deposited from the National Tuberculosis Reference Laboratory of Iran between 2011 and 2018. Methods: The study evaluated the prevalence of NTM among specimens from patients with pulmonary tuberculosis symptoms (n=15 771) deposited at the National Tuberculosis Reference Laboratory of Iran from 2011 to 2018. Detection of Mycobacterium (M.) tuberculosis was based on presence of a 190-bp amplicon from IS6110 insertion sequence using Tb1 and Tb2 primers, and amplicon-negative specimens were tested for NTM and M. tuberculosis (refractory to IS6110 amplification) using restriction fragment length polymorphism PCR of hsp65 amplicon fragment. Results: A total of 7 307 (46.33%) M. tuberculosis and 658 (4.17%) NTM specimens were found, the latter mainly comprising M. abscessus (10.18%), M. avium (2.28%), M. chelonae (8.97%), M. intracellulare (10.49%), M. kansasii (4.71%), and M. simiae (56.08%). Conclusions: As treatment for NTM differs from that for M. tuberculosis, accurate detection of Mycobacterium sp. is of public health significance.


How to cite this article:
Ayoubi S, Farnia P, Farnia P, Aghajani J, Ghanavi J, Velayati AA. Prevalence of non-tuberculosis mycobacteria among samples deposited from the National Tuberculous Reference Laboratory of Iran (2011-2018).Asian Pac J Trop Med 2021;14:451-455


How to cite this URL:
Ayoubi S, Farnia P, Farnia P, Aghajani J, Ghanavi J, Velayati AA. Prevalence of non-tuberculosis mycobacteria among samples deposited from the National Tuberculous Reference Laboratory of Iran (2011-2018). Asian Pac J Trop Med [serial online] 2021 [cited 2021 Dec 5 ];14:451-455
Available from: https://www.apjtm.org/article.asp?issn=1995-7645;year=2021;volume=14;issue=10;spage=451;epage=455;aulast=Ayoubi;type=0