Asian Pacific Journal of Tropical Medicine

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 11  |  Issue : 4  |  Page : 280--284

DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes


Jean Noël Nyemb1, Roland Ndoubalem3, Emmanuel Talla3, Alembert Tiabou Tchinda2, Dieudonné Ndjonka3, Celine Henoumont4, Sophie Laurent4, Joseph Tanyi Mbafor5 
1 Department of Organic Chemistry, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde; Department of Chemistry, Faculty of Science, University of Ngaoundere, P.O. Box 454, Ngaoundere, Cameroon
2 Institute of Medical Research and Medicinal Plants Studies (IMPM), Ministry of Scientific Research and Innovation, P.O. Box 6163 Yaounde, Cameroon
3 Department of Biology, Faculty of Science, University of Ngaoundere, P.O. Box 454, Ngaoundere, Cameroon
4 Department of General, Organic and Biomedical Chemistry, Faculty of Medicine and Pharmacy, University of Mons, NMR and Molecular Imaging Laboratory, B-7000 Mons, Belgium
5 Department of Organic Chemistry, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon

Correspondence Address:
Jean Noël Nyemb
Department of Chemistry, Faculty of Science, University of Ngaoundere, P.O Box 454, Ngaoundere
Cameroon

Objective: To investigate the phytochemical constituents, antioxidant and anthelmintic activities of the crude methanol extract of Cissus populnea (C. populnea) rhizomes. Methods: Phytochemical screening was performed using standard protocols, and column chromatography of silica gel was used for the compounds isolation. DPPH antiradical scavenging assay was performed in order to evaluate the antioxidant activity. Total phenolic content was evaluated using the Folin–Ciocalteu assay. The anthelmintic activity was screened on the bovine adult male forms of parasitic nematode Onchocerca ochengi, by the in vitro evaluation of the inhibition of adult worm motility and mortality. Worms were incubated in the presence of different concentrations of the plant extract and effects on survival were monitored after 24 and 48 h. Results: The preliminary phytochemical screening revealed the presence of phenolic compounds, saponins, steroids, tannins, and terpenoids. Bergenin and a mixture of phytosterol, β -sitosterol and stigmasterol were isolated from this extract and were identified by nuclear magnetic resonance, mass spectrometry and by comparison with published data. The crude methanol extract of C. populnea rhizomes showed a strong DPPH antiradical activity with a good amount of total phenolic content ((20.69±2.13) g gallic acid equivalent/100 g of extract) and significant anthelmintic activity comparable to the standard drug ivermectin. Bergenin was found to be inactive even after 72 h of incubation. Conclusions: This study constitutes the first report on the anthelmintic activity of this plant and supports the traditional use of C. populnea as a natural antioxidant and anthelmintic.


How to cite this article:
Nyemb JN, Ndoubalem R, Talla E, Tchinda AT, Ndjonka D, Henoumont C, Laurent S, Mbafor JT. DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes.Asian Pac J Trop Med 2018;11:280-284


How to cite this URL:
Nyemb JN, Ndoubalem R, Talla E, Tchinda AT, Ndjonka D, Henoumont C, Laurent S, Mbafor JT. DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes. Asian Pac J Trop Med [serial online] 2018 [cited 2022 May 25 ];11:280-284
Available from: https://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=4;spage=280;epage=284;aulast=Nyemb;type=0