Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley, Malaysia

Nurul Diana Dzaraly; Mohd Nasir Mohd Desa; AbdulRahman Muthanna; Siti Norbaya Masri; Niazlin Mohd Taib; Nurul Hana Zainal Baharin; Nurshahira Sulaiman; Nurul Asyikin Abdul Rahman; Navindra Kumari Palanisamy; Zarizal Suhaili; Semiat Opeyemi Yusuff; Nor Iza A. Rahman; Tuan Suhaila Tuan Soh; Fatimah Haslina Abdullah8

doi:10.4103/1995-7645.370150

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ORIGINAL ARTICLE

Year : 2023 \| Volume : 16 \| Issue : 2 \| Page : 80-88

Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley, Malaysia Nurul Diana Dzaraly¹, Mohd Nasir Mohd Desa¹, AbdulRahman Muthanna¹, Siti Norbaya Masri², Niazlin Mohd Taib², Nurul Hana Zainal Baharin¹, Nurshahira Sulaiman¹, Nurul Asyikin Abdul Rahman³, Navindra Kumari Palanisamy⁴, Zarizal Suhaili⁵, Semiat Opeyemi Yusuff², Nor Iza A. Rahman⁶, Tuan Suhaila Tuan Soh⁷, Fatimah Haslina Abdullah8⁸ ¹ Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia ² Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia ³ Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, UPM Serdang, Selangor; School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA Negeri Sembilan, Kuala Pilah Branch, Kuala Pilah, Negeri Sembilan, Malaysia ⁴ Department of Medical Microbiology and Parasitology, Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, Jalan Hospital, Sungai Buloh, Selangor, Malaysia ⁵ School of Animal Science, Aquatic Science and Environment, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, Besut Campus, Besut, Terengganu, Malaysia ⁶ Faculty of Medicine, Universiti Sultan Zainal Abidin, Jalan Sultan Mahmud, Kuala Terengganu, Terengganu, Malaysia ⁷ Department of Pathology, Sungai Buloh Hospital, Jalan Hospital, Sungai Buloh, Selangor, Malaysia ⁸ Department of Pathology, Sultanah Nur Zahirah Hospital, Jalan Sultan Mahmud, Kuala Terengganu, Terengganu, Malaysia Correspondence Address: Mohd Nasir Mohd Desa Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, UPM Serdang, Selangor Malaysia Source of Support: This study was supported by Fundamental Research Grant Scheme (FRGS/1/2020/SKK0/UPM/02/10) of the Ministry of Higher Education Malaysia, Conflict of Interest: None DOI: 10.4103/1995-7645.370150

Objective: To characterise a collection of pili-carrying and none pili-carrying pneumococcal isolates of clinical origin for serotypes, antibiotic resistance and genotype. Methods: In total, 42 clinical isolates were collected between October 2017 and December 2019. Those isolates were analysed for antimicrobial susceptibility, serotype distribution, detection of pneumococcal virulence and pilus genes. Multilocus sequence typing was performed only for piliated isolates, followed by phylogenetic analysis. Results: The common isolation sites among the pneumococcal isolates were tracheal aspirate (28.6%), blood (26.2%), and sputum (23.8%). Fifty percent isolates were resistant to erythromycin, tetracycline (50.0%) and trimethoprim-sulfamethoxazole (43.0%). The most frequent were serotypes 19F (28.6%), 6A/B (23.8%) and 19A (14.3%). Piliated isolates were detected in a small proportion (33.3%); 64.3% were multidrug-resistant. ST320 was the prevalent sequence type among the piliated isolates and genetically related to the Pneumococcal Molecular Epidemiology Network clones Taiwan^19F-14 (CC271). In the phylogenetic analysis, some piliated isolates showed a close association having similar ST320, carrying serotype 19A and both pilus genes indicating their clonal spread. Conclusions: Pneumococcal lineages of piliated isolates have been globally disseminated and pili could have played a role in the spread of antibiotic resistant clones.



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Pneumococci; Pili; Genotype; Malaysia

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