| SHORT COMMUNICATION |
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| Year : 2020 | Volume
: 13
| Issue : 10 | Page : 464-466 |
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Performance and correlation of interferon gamma release assays and tuberculin skin test in HIV-infected children and adolescents with immune reconstitution
Keswadee Lapphra1, Rati Diwitaya1, Sansnee Senawong2, Maneeprang Thovarabha2, Parnwas Pinnobphun3, Alan Maleesatharn1, Nantaka Kongstan1, Benjawan Khumcha1, Wanatpreeya Phongsamart1, Orasri Wittawatmongkol1, Supattra Rungmaitree1, Kulkanya Chokephaibulkit1
1 Department of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
2 Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
3 Biomed Diagnostics (Thailand) Co LTD, Bangkok, Thailand
Correspondence Address:
Kulkanya Chokephaibulkit
Department of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok
Thailand
 Source of Support: This study was supported by the Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand, [Grant Number (IO) R015832028)]. Oxford Immunotec and Biomed diagnostics (Thailand) provided the T-SPOT.TB test kit, Conflict of Interest: None
DOI: 10.4103/1995-7645.291040
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Objective: To evaluate the performance of interferon gamma release assays and tuberculin skin test in HIV-infected children and adolescents with immune reconstitution.
Methods: A cross-sectional study was conducted in HIV-infected patients aged 5-18 years receiving antiretroviral treatment with CD4 T-lymphocytes >25% or >500 cells/mm3 for at least 6 months. QuantiFERON-TB Gold, T-SPOT.TB, and tuberculin skin test were performed in each patient.
Results: A total of 50 patients were enrolled with median age of 13.7 years, CD4 counts of 753 (IQR: 587-989) cells/mm3. Among 27 patients with tuberculosis (16) or tuberculosis exposure (11), 8 (29.6%) were positive to at least one test, 2 (7.4%) were positive QuantiFERON-TB Gold, 3 (11.1%) positive T-SPOT.TB, and 7 (25.9%) had tuberculin skin test ≥5 mm. Among 23 patients without history of tuberculosis or exposure, all had negative interferon gamma release assays, while 2 (8.7%) had positive tuberculin skin test.
Conclusions: All tests had low sensitivity despite immune reconstitution. |
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