|Year : 2020 | Volume
| Issue : 10 | Page : 456-463
Pomegranate peel extract lessens histopathologic changes and restores antioxidant homeostasis in the hippocampus of rats with aluminium chloride-induced Alzheimer’s disease
Steve Harakeh1, Wafaa S Ramadan2, Mohammed S Al Muhayawi3, Soad Al Jaouni4, Shaker Mousa5, Khalid Rehman Hakeem6
1 Special Infectious Agent Unit, King Fahd Medical Research Center; Yousef Abdul Latif Jameel Scientific Chair of Prophetic Medicine Application, Faculty of Medicine, King Abdulaziz University, Kingdom of Saudi Arabia
2 Department of Anatomy, Faculty of Medicine, King Abdulaziz University, Kingdom of Saudi Arabia; Department of Anatomy, Faculty of Medicine, Ain Shams University, Cairo, Egypt
3 Department of Medical Microbiology/Parasitology and Molecular Microbiology Laboratory, KAUH; Department of Hematology/Pediatric Oncology, King Abdulaziz University Hospital (KAUH), Faculty of Medicine, King Abdulaziz University, Kingdom of Saudi Arabia
4 Yousef Abdul Latif Jameel Scientific Chair of Prophetic Medicine Application, Faculty of Medicine, King Abdulaziz University, Kingdom of Saudi Arabia; Pharmaceutical Research Institute, Albany College of Pharmacy and Health Sciences, Rensselaer, NY, USA
5 Yousef Abdul Latif Jameel Scientific Chair of Prophetic Medicine Application, Faculty of Medicine, King Abdulaziz University, Kingdom of Saudi Arabia
6 Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia
|Date of Submission||26-May-2019|
|Date of Decision||25-May-2020|
|Date of Acceptance||02-Jun-2020|
|Date of Web Publication||14-Aug-2020|
Khalid Rehman Hakeem
Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Jeddah
Source of Support: None, Conflict of Interest: None
Objective: To investigate the neuroprotective efficacy of pomegranate and ellagic acid on the histopathological changes in the hippocampus of an aluminium chloride (AlCl3) induced rat model of Alzheimer’s disease.
Methods: Sprague Dawley rats were divided into 4 groups (n=10 each): Group I : serving as negative control; Group II, Alzheimer model, induced by administration of 17 mg/kg bw AlCl3; Group III, administered the same dose of AlCl3 with 50 mg/kg of pomegranate peel extract and Group IV : administered ellagic acid (50 mg/kg) in addition to the same dose of AlCl3. The medication given to all groups continued for 28 days. All were given the compounds by gastric gavage. Radial arm maze test, hippocampus antioxidant markers, histopathology of the dentate gyrus, and CA3 of the hippocampus were evaluated.
Results: Rats treated with pomegranate peel extract exposed to radial arm maze test showed less number of errors and reduced time needed to reach the criterion. There was an increase in the levels of glutathione, catalase, and total antioxidant capacity and decreased lipid peroxidation products. Histopathological features in dentate gyrus and CA3 as apoptosis and chromatolysis of pyramidal cells and granular layer, respectively, were decreased. Alzheimer characteristic neurofibrillary tangles and senile plaques were reduced. Treatment with ellagic acid ameliorated the pathological results but to a statistically lower level.
Conclusions: Pomegranate peel extract alleviates memory deficit and restores antioxidant homeostasis following degenerative changes in the hippocampus induced by aluminium chloride in rats.
Keywords: Pomegranate; Peel extract; Ellagic acid; Antioxidants; Hippocampus; Behavioural test
|How to cite this article:|
Harakeh S, Ramadan WS, Al Muhayawi MS, Al Jaouni S, Mousa S, Hakeem KR. Pomegranate peel extract lessens histopathologic changes and restores antioxidant homeostasis in the hippocampus of rats with aluminium chloride-induced Alzheimer’s disease. Asian Pac J Trop Med 2020;13:456-63
|How to cite this URL:|
Harakeh S, Ramadan WS, Al Muhayawi MS, Al Jaouni S, Mousa S, Hakeem KR. Pomegranate peel extract lessens histopathologic changes and restores antioxidant homeostasis in the hippocampus of rats with aluminium chloride-induced Alzheimer’s disease. Asian Pac J Trop Med [serial online] 2020 [cited 2022 Jul 3];13:456-63. Available from: https://www.apjtm.org/text.asp?2020/13/10/456/291039
| 1. Introduction|| |
Alzheimer’s disease (AD), a neurodegenerative brain disorder, is the most common cause of dementia,. Cumulative oxidative stress has been attributed to its etiology. Excessive production of oxidants in the human body can lead to oxidative damage to large biomolecules such as lipids, DNA, and proteins. The hippocampus is extensively susceptible to oxidative stress. Knowing that it plays an important role in spatial cognition, representing the elements of everyday interactions and the relations among them marked the hippocampus as a pivot in-memory processing.
Cognitive dysfunction has been found to be among the most sensitive impairments associated with exposure to environmental contaminants in human and experimental animal models. Previous studies have indicated that the presence of aluminium in drinking water and foods lead to AD in humans and animals,. Aluminium causes microtubule depletion, eventually leading to disconnection of the neuronal afferents and efferents, loss of function and regional atrophy, which is consistent with MRI findings in AD brains. It is well documented that Al-based compounds are widely used in many fields like medicine, industries, water purification tool and household cookware.
Oxidative stress is associated with many pathological conditions affecting the nervous system, many compounds have been demonstrated, based on trials, to decrease the cellular load of reactive oxygen species. Currently prescribed medications for the treatment of AD break the vicious oxidative stress cycles and neurodegeneration. However, they may compromise the signal transduction pathways and prevent free radical production. Meanwhile, the antioxidant products, present in fruits, vegetables and medicinal plants, have become the centre of attention as they proved their defensive role,.
Pomegranate fruit (Punica granatum L.) displayed many therapeutic effects on using extracts of all its parts (peel, seeds, juice),. This has been attributed to various reasons, especially due to their antioxidant properties and their total polyphenol activity. The peel exhibited a high content of polyphenols of which ellagitannins are the major constituents, and are further hydrolyzed into ellagic acid in the gut. Ellagitannins are also known for their antioxidant effect. The availability of free ellagic acid in food is unusual; it is conjugated with either a glycoside moiety or forms part of the polymeric ellagitannins. The beneficial role of using the whole herb or combination of a group of herbs aims at several objectives; it will mitigate the toxicity and reduce the side effects of one, isolated compound from the plant. Many previous studies addressed the high nutritional and potential role of an extract of Punica granatum. Proofs are accumulating that compounds present in a fruit or herb extract augment each other’s biological effect.
The aim of the present study was to compare the antioxidant effect of the total pomegranate peel extract with its single principle constituent, ellagic acid, on the neurodegenerative effects in the hippocampus rats and decreasing the incidence of subsequent resulting dementia.
| 2. Material and methods|| |
Forty Sprague Dawley rats with an body weight of 200-250 g were purchased from Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia. The rats were housed in wire-meshed cages at a constant temperature (22±2) °C, 55% humidity and light-dark conditions (12/12 hours light/dark). Animals were provided with Purina chow diet (Purina, Ontario) and drinking water ad libitum and left to habituate to the laboratory environment for one week. The protocol of the current study followed the guidelines of animal care at Faculty of Pharmacy Animal House, KAU. Ethical approval No. 330-19 was granted by ethical committee at King Abdulaziz University, Jeddah, Saudi Arabia.
Aluminium chloride (AlCl3) and ellagic acid (EA) were purchased from Sigma Aldrich. Standardized pomegranate extract (PE) was POMELLA™ (Verdure Sciences, Noblesville, IN, USA), which has been studied in human subjects for its safety/tolerability and bioavailability.
2.2. Experimental design
The rats were randomly divided into four groups (n=10 each). Group I (control): was the negative control and received only food and water. Group II (AD) received AlCl3 administered orally at a dose of 17 mg/kg body weight (bw) daily for 28 days. Both group III and IV were also given AlCl3 at a dose of 17 mg/kg body weight (b.w.) daily for 28 days. Then group II (AD+PE) received PE 50 mg/kg for 28 days. Group IV (AD+EA) received EA 50 mg/kg dissolved in DMSO for 28 days. Treatment was given to the latter two groups by intragastric tube daily after AlCl3 administration was stopped.
2.2.1. Behavioural tests: Radial arm maze (RMT)
A 12 arm radial maze was built to be similar to that used by Lai et al. Rats were habituated to the maze 10 min daily for 4 days. This was followed by the test phase (14 days) in which the rat searched the maze for food pellets present at the end of each of the 12 arms. The rat was kept in the maze until it entered all 12 arms or 10 min had passed. The maze was cleaned entirely before each test session. An entry was considered when all four paws were inside an arm. Two main variables were recorded: the number of errors and the time to complete the maze. Errors are defined as when a rat chooses an unbaited arm (reference memory error) or when it returns to a baited arm after obtaining the food reward (working memory error). The time it took for each rat to enter the 12 baited arms was measured and considered as time to criterion.
2.2.2. Brain tissue sampling
At the end of the experiment, the rats were deprived of food for 12 h then sacrificed by decapitation under humane conditions. The whole brain was rapidly extracted, washed with isotonic saline, dried, and sagittally divided into two halves; the hippocampus of the left side was dissected for homogenate preparation and the right-sided cerebral hemisphere for histopathological examination.
2.2.3. Preparation of hippocampus homogenate
Hippocampus homogenization was done as previously published.
2.2.4. Biomarkers for antioxidant activity
Estimation of catalase activity was performed according to Chance and Maehly. The activity of catalase was expressed as % activity. Evaluation of glutathione in the hippocampus was done following the method of Habiget et al. The concentration was expressed as μmol per mg protein. The TAC in brain homogenate was determined using Cell Biolabs’ OxiSelect™ TAC Assay (Catalog Number STA-360). The reaction was read with a standard 96-well spectrophotometric microplate reader at 490 nm.
2.2.5. Estimation of lipid peroxidation assay: Thiobarbituric acid reactive substance
The assay for lipid peroxidation was carried out by assessing the thiobarbituric acid reactive substance following Ohkawa et al. Results were expressed as μM of malondialdehyde (MDA) per mg protein.
2.2.6. Histopathological examination
The second portion of each brain was fixed in phosphate buffer formalin (10%) for 24 h. The brains were processed and embedded in paraffin blocks. Sections (4 μm thick) were stained with hematoxylin and eosin stains, Cresyl violet, and Silver stains. Sections were examined and photographed using an Olympus light microscope (model: BX51TF-Japan).
2.2.7. Morphometric analysis
Both the thickness of the pyramidal cell layer in the CA3 area and the granular cell layer of the dentate gyrus (DG) were measured using Image ProPlus Software, (Media Cybernetics, Silver Spring, MD). Five non-overlapping fields in each of 10 sections for each rat were assessed and the mean for each of the ten rats was calculated.
2.2.8. Statistical analysis
Statistical analyses were performed using SPSS program version 20 (IBM-USA). The one-way analysis of variance (ANOVA) test was used and when equal variance could be assumed, the LSD t-test was applied. Data are presented as mean±standard deviation (mean±SD). P<0.05 was considered statistically significant.
| 3. Results|| |
3.1. Behavioral test
In the RMT, the number of errors in 14 days’ time significantly decreased in the groups treated with PE and EA in comparison to the AD group. Rats treated with PE made fewer errors than those treated with EA [Figure 1]A. Through out the phase test (14 days), it was documented on daily bases that the time spent by control rats to reach criterion was significantly shorter than those of the AD group (P<0.05). Rats treated with PE and EA significantly decreased the time to reach criterion (P<0.05) in comparison to the AD group [Figure 1]B.
|Figure 1: Line graph showing (A) the mean number of errors made by rats in each of the four groups for each daily test trial and the mean time to criterion (12 arms); (B) n=10 rats/group. Data were expressed as mean±SD. Cont: control, AD: Alzheimer model, AD+PE: treated with pomegranate peel extract, AD+EA: treated with ellagic acid. *P<0.05 indicates a significant difference from the control. #P<0.05 indicates a significant difference from AD group.|
Click here to view
3.2. Biomarkers for the antioxidant activity
In comparison with the control group, the concentration of catalase and glutathione in the hippocampus homogenate significantly decreased in the AD rat model to (110.41±4.80) ng/mL and (23.12±3.30) μM, respectively. On the other hand, treatment with PE enhanced the antioxidant activity and resulted in the higher catalase and glutathione levels in comparison to those rats on EA. Moreover, the TAC yielded by PE was significantly higher than those administered EA [Table 1].
|Table 1: Catalase, glutathione activity, total antioxidant capacity (TAC) and lipid peroxidation products (MDA) in all groups.|
Click here to view
3.3. Estimation of lipid peroxidation assay: Thiobarbituric acid reactive substance (TBARS)
In comparison to AD group, the supplementation of EA decreased MDA by 15.4% while PE administration reduced it by 28.4%. PE therapy significantly decreased the level of MDA from (41.50± 3.20) μM in the AD group to (29.7±1.60) μM [Table 1].
3.4. Histopathological results
Examination of the CA3 region of the hippocampus of the control group using H&E stain revealed poly-morphic, pyramidal and molecular cell layers. The pyramidal cells, with large vesicular nuclei and basophilic cytoplasm, were packed in 4-5 layers [Figure 2]A. Cresyl violet staining showed the well-defined distribution of Nissl’s granules corresponding to rough endoplasmic aggregates, giving characteristic blue hallow surrounding the nucleus [Figure 2]B. Silver staining of the control sections showed round polyhedral pyramidal cells [Figure 2]C.
|Figure 2: Photomicrographs of sections of hippocampal CA3 region showing normal poly-morphic (PO), pyramidal (PY) and molecular cell layers (MO) in the control group (A) and well defined distribution of nissl granules in (PY) cells (B). Silver stain showing normal pyramidal cells (C). In AD, apparent decreased thickness of the (PY) layer, pyramidal cells are shrunk with pyknotic nuclei (arrows), with vacuolated cytoplasm and areas devoid of cells are also revealed (D). Marked chromatolysis of PY cells (E), NFT (thin arrows) and (SP) (thick arrows) are prominent (F). In rats treated with PE, most of pyramidal cells of CA3 show normal structure (G), restored nissel granules (H) and less NFT and SP are noted (I). In groups treated with AD+EA, frequent apoptotic cells (arrows) are noted (J), chromatolysis of pyramidal cells (K) and persistent NFT and SP are still noted (L). AD: Alzheimer, NFT: neurofibrillar tangles, SP, senile plaques, PE: pomergrantae peel extract, EA: ellagic acid, (A, D, G, Jx H&E×400), (B, EH, Kx Cresyl violet ×400), (C, F, I, Lx Silver×400).|
Click here to view
In the AD group, some of the pyramidal cells appeared shrunk with pyknotic nuclei, others had vacuolated cytoplasm. Microvacuolation of the neuropil with areas devoid of cells were also revealed [Figure 2]D. Cresyl violet stain showed marked chromatolysis, which explains the vacuolated appearance of pyramidal cells in H&E stain [Figure 2]E.
The pathological hallmarks of AD were revealed in silver stain; (Neurofibrillary tangles) NFT and senile plaques (SP) [Figure 2]F. Rats treated with PE decreased the degenerative changes; NFT and SP. The Nissl’s granules and the mean thickness of the pyramidal layer were restored [Figure 2]G, [Figure 2]H, [Figure 2]I. Frequent NFT, SP, and other degenerative changes persisted in sections of rats treated with EA. [Figure 2]J, [Figure 2]K, [Figure 2]L. Decreased mean thickness of the pyramidal layer [Figure 3] reflected the degenerative changes in this layer.
|Figure 3: The mean thickness of the pyramidal layer in groups treated with AD+PE in comparison to AD and groups treated with AD+EA. The one-way analysis of variance (ANOVA) test was used. The Fisher's least significant difference (LSD) t-test was applied when equal variance could be assumed. Data are presented as means±SD. *significantly different from the control, AD+PE, AD+EA at P≤0.05. #significantly different from AD group at P≤0.05.|
Click here to view
The DG consisted of the molecular, granular cell, and pleomorphic layers. The granular cell layer of the control group contained polyhedral cells with vesicular nuclei, with well-dispersed Nissl’s granules manifested in cresyl violet stain and large polyhedral cells by silver stain [Figure 4]A, [Figure 4]B & [Figure 4]C. In the AD model, reduced thickness of granular cell layer, marked vacuolation of disorganized granular cells, with multiple shrunk cells and pyknotic nuclei were all revealed in H&E stain [Figure 4]D. Marked chromatolysis with a peripheral displacement of the nucleus [Figure 4]E and multiple SP and NFT were dispersed in the granular layer [Figure 4]F manifested in cresyl stain and silver stain, respectively. These degenerative changes were ameliorated on treatment with PE [Figure 4]G, [Figure 4]H & [Figure 4]I but persisted on treatment with EA [Figure 4]J, [Figure 4]K & [Figure 4]L. A significant decrease in the mean thickness of the granular layer corresponded with degenerative changes [(55.0±3.40) pm] in comparison to the control [(79.0±1.50) pm] [Figure 5].
|Figure 4: Photomicrographs of sections of hippocampal DG region showing normal molecular (M), granular cell (G) and pleomorphic layers (P) in the control group (A) and prominent nissl granules in (B), and large polyhydral cells (C). In AD model, reduced thickness of granular cell layer, marked vacuolation of disorganized cells (dashed arrows), and multiple shrunken cells with pyknotic nuclei (arrows) are noted (D). Chromatolysis of (G) cells (dashed arrows) (E) and multiple SP (arrows) and NFT (dashed arrows) are dispersed (F). Signs of degenerative changes decreased with PE administration (G-I) more than with EA treatment (J, K, L). DG: dentate gyrus; AD: Alzheimer; NFT: neurofibrillar tangles; SP: senile plaques; PE: pomergrantae peel extract; EA: ellagic acid (A, D, G, Ix H&E ×400), (B, E, H, Kx cresyl violet×400), (C, F, I, Lx Silver×400).|
Click here to view
|Figure 5: The mean thickness of the granular layer on treatment with PE. The one-way analysis of variance (ANOVA) test was used. The Fisher's least significant difference (LSD) t-test was applied when equal variance could be assumed. Data are presented as means±SD. *significantly different from the control, AD+PE, AD+EA at P≤0.05. #significantly different from AD group at P≤0.05.|
Click here to view
| 4. Discussion|| |
Several studies agree that body cells exposed to oxidative stress at the physiological state and subjected to factors like stress, ageing, and environmental degenerative contaminants reinforce the damage caused by oxidative stress. This work aims to compare the antioxidant effect of whole peel PE with its single active component, EA, in relation to neurodegenerative histopathological changes in the hippocampus of rat brains in an AD model. The hippocampus plays an exclusive role in learning and memory and is subjected to early affection in AD. The hippocampus is formed of DG and the hippocampus proper, which is further composed of CA1, CA2, and CA3 fields and the subiculum.
Neurodegenerative changes in hippocampus exposed to AlCl3 revealed reduced thickness of granular cells in DG and pyramidal cell in CA3 in addition to vacuolation of cells and pyknosis of their nuclei. Chromatolysis and loss of Nissl’s granules were evident in cresyl violet stained sections. Similar results were described by Damjanov as signs of neuronal injury. The darkly stained fibrillary tangles NFT involving the neurons and focal interneuronal spherical neuritic plaques SP were also described as pathological hallmarks of AD. The aggregation and formation of NFT resulted from hyperphosphorylation of Tau protein, which dis-stabilises neuron microtubules as analyzed by Rodríguez-Martín et al and resulted in neurodegeneration and loss of function. It is confirmed that oxidative damage in the brain precipitated neurofibrillary tangle NFT. The SP resulted in the abnormal deposition of the amyloid-β peptide (Aβ) with 40 or 42 amino acids (Aβ40 and Aβ42) outside the cells, which are the metabolism by-products of the amyloid precursor protein (APP).
There is evidence suggesting an interconnection between oxidative stress and tau pathology. It has been reported that neurons with excessive tau protein showed increased susceptibility to oxidative stress, and the consumption of peroxisomes was the reason. Moreover, accumulation of extracellular amyloid plaques led to increased oxidative stress, induced mitochondrial dysfunction and increased the levels of the byproducts of lipid peroxidation.
In the present study oxidative stress was proved to be a factor in AD neurodegenerative changes as manifested by decreased catalase, glutathione, TAC, and increased lipid peroxidation products (MDA). This was reflected in rats undergoing the RMT. AD rat model revealed impairment of spatial memory as the number of errors increased while time to reach criterion decreased. This is supported by results from a study by Mohsen et al.
Previous research generally confirmed that RMT could be used to test for the reference and working memory performance simultaneously. It has been proven that the DG integrates both the visual object and spatial information to create a memory of a ‘total’ spatial representation, while the CA3 region plays a role in pattern completion or the retrieval of memory when only partial input cues are presented.
In the present study, the PE mitigated the neurodegenerative pathological changes in the hippocampus more than EA. This was manifested by regaining thickness of the granular layer in DG and pyramidal layer in CA3, the decrease in NFT and SP formation, and restoration of Nissl’s granules. This can be attributed to its antioxidant effect revealed in elevation of the lowered levels of catalase, glutathione and lowering of the high levels of lipid peroxidation products.
PE relieved histopathological changes in the AD model better than EA. These results were supported by the stronger antioxidant effect manifested by a statistically significant TAC in PE more than in EA treated group. It is documented that pomegranate peel ethanolic extract exhibited proton-donating ability, demonstrated the highest free radical scavenging activity, and had the highest inhibitory activity on lipid peroxidation.
The peel comprises about 50% of the total fruit weight and is considered an indispensable source of bioactive compounds such as phenolics, flavonoids, ellagitannins, and proanthocyanidin compounds, minerals, and complex polysaccharides.
In conclusion, it was found that therapeutic potential of pomegranate peel extract on the neurodegenerative changes induced in the hippocampus of rats and the subsequent resulting dementia was better than the effect of a single principle constituent, ellagic acid.
Conflict of interest statement
The authors declare no conflict of interest among them.
This project was funded by Deanship of Scientific Research (DSR), King Abdulaziz University, Jeddah, under grant No. DF-658-248- 1441). The authors, therefore, gratefully acknowledge DSR technical and financial support.
S.T. supervised the project, W.R. designed the study and carried out the experiment. M.M. contributed in writing the manuscript and literature search, S.J. and S.M. analyzed and interpreted the collected data. S.T. and K.R. supported the study through critical comments and reviewing of the manuscript.
| References|| |
Wimo A, Jonsson L, Winblad B. An estimate of the worldwide prevalence and direct costs of dementia in 2003. Dement Geriatr Cogn Dis
Blennow K, de Leon MJ, Zetterberg H. Alzheimer’s disease. Lancet
Song P, Zou MH. Roles of reactive oxygen species in physiology and pathology. In: Wang H, Patterson C, editors. Atherosclerosis: Risks, mechanisms, and therapies
. Hoboken, NJ: John Wiley & Sons Inc.; 2015, p. 379-392.
Poulose SM, Miller MG, Shukitt-Hale, B. Role of walnuts in maintaining brain health with age. J Nutr
(Suppl 4): S561-S566.
Gottlieb M, Leal-Campanario R, Campos-Esparza MR, Sanchez-Gomez MV, Alberdi E, Arranz A, et al. Neuroprotection by two polyphenols following excitotoxicity and experimental ischemia. Neurobiol Dis
Deacon RM, Rawlins JNP. Hippocampal lesions, species-typical behaviours and anxiety in mice. Behav Brain Rer
Eskenazi B, Marks AR, Bradman A, Harley K, Barr DB, Johnson C, et al. Organophosphate pesticide exposure and neurodevelopment in young Mexican-American children. Environ Health Perspect
Levin ED, Addy N, Baruah A, Elias A, Christopher NC, Seidler FJ, et al. Prenatal chlorpyrifos exposure in rats causes persistent behavioral alterations. Neurotoxicol Teratol
Exley C. The aluminium-amyloid cascade hypothesis and Alzheimer’s disease. Alzheimer’s disease
. Boston: Springer; 2005, p. 225-234.
Walton JR. Chronic aluminum intake causes Alzheimer’s disease: Applying Sir Austin Bradford Hill’s causality criteria. J Alzheimer’s Dis
Lione A. Aluminum toxicology and the aluminum-containing medications. Pharmacol Therap
Abbasali K, Zhila T, Farshad N. Developmental toxicity of aluminum from high doses of AlCl3
in mice. J Appl Res
Newairy ASA, Salama AF, Hussien HM, Yousef MI. Propolis alleviates aluminium induced lipid peroxidation and biochemical parameters in male rats. Food Chem Toxicol
Bilkei-Gorzo A. Neurotoxic effect of enteral aluminium. Food Chem Toxicol
Slemmer JE, Shacka JJ, Sweeney MI, Weber JT. Antioxidants and free radical scavengers for the treatment of stroke, traumatic brain injury and aging. Curr Med Chem
Gella A, Durany N. Oxidative stress in Alzheimer disease. Cell Adh Migr
Fu L, Xu BT, Xu XR, Gan RY, Zhang Y, Xia EQ, et al. Antioxidant capacities and total phenolic contents of 62 fruits. Food Chem
Deng GF, Xu XR, Guo YJ, Xia EQ, Li S, Wu S, et al. Determination of antioxidant property and their lipophilic and hydrophilic phenolic contents in cereal grains. J Funct Food
Newman RA, Lansky EP, Block ML. Pomegranate: The most medicinal fruit
. Laguna Beach, California: Basic Health Publications; 2007, p. 120.
Jurenka J. Therapeutic applications of pomegranate (Punica granatum
L.): A review. Alter Med Rev
Tzulker R, Glazer I, Bar-Ilan I, Holland D, Aviram M, Amir R. Antioxidant activity, polyphenol content, and related compounds in different fruit juices and homogenates prepared from 29 different pomegranate accessions. J Agric Food Chem
Faria A, Calhau C. The bioactivity of pomegranate: Impact on health and disease. Critical Rev Food Sci Nut
Gil MI, Tomas-Barberan FA, Hess-Pierce B, Holcroft DM, Kader AA. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. J Agricul Food Chem
Larrosa M, Tomás-Barberán FA, Espín JC. The dietaryhydrolysable tannin punicalagin releases ellagic acid that inducesapoptosis in human colon adenocarcinoma Caco-2 cells by usingthe mitochondrial pathway. J Nutr Biochem
Jain S, Rai R, Upadhyaya AR, Malhotra G. Punica granatum:
A natural and recent approach towards dental problem. Intern J Pharma Sci Res
Mertens-Talcott SU, Jilma-Stohlawetz P, Rios J, Hingorani L, Derendorf HJ. Absorption, metabolism, and antioxidant effects of pomegranate (Punica granatum
L.) polyphenols after ingestion of a standardized extract in healthy human volunteers. Agric Food Chem
Toklu HZ, Dumlu MU, Sehirli O, Ercan F, Gedik N, Gokmen V, et al. Pomegranate peel extract prevents liver fibrosis in biliaryobstructed rats. J Pharm Pharmacol
Li Y, Mu Y, Gage FH. Development of neural circuits in the adult hippocampus. Curr Top Dev Biol
Genovese RF, Benton BJ, Shippee SJ, Jakubowski EM, Bonnell JC. Effects of low level inhalation exposure to cyclosarin on learned behavior in Sprague Dawly rats. J Toxicol Environ Health
Kumar P, Kumar A. Neuroprotective effect of cyclosporine and FK506 against 3-nitropropionic acid induced cognitive dysfunction and glutathione redox in rat: Possible role of nitric oxide. Neurosci Res
Chance B, Maehly AC. Assay of catalase and peroxidases. Met Enzymol
Habig WH, Pabst MJ, Jakoby WB. Glutathione-S-transferases: The first enzymatic step in mercapturic acid formation. J Biol Chem
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem
Bancroft JD, Stevens A. The haematoxylin and eosin. In: Theory and practice of histological techniques
. 4th ed. London, New York & Tokyo: Churchill Livingstone; 1996, p. 99-112.
Halliwell B, Gutteridge JMC. Cellular responses to oxidative stress: Adaptation, damage, repair, senescence and death. In: Free radicals in biology and medicine
. 4th ed. Oxford UK: Clarendon Press; 2007, p. 187-267.
Damjanov I. Central nervous system. In, Paul St. Romain, Damjanov, editors. In: Atlas of histopatholgy
. 1st edition. New Delhi: Joypee Brothers Medical Publishers(P) Ltd.; 2012, p. 367-372.
Serrano-Pozo A, Frosch MP, Masliah E, Hyman BT. Neuropathological alterations in Alzheimer disease. Cold Spring Harb Perspect Med
Rodríguez-Martín T, Cuchillo-Ibáñez I, Noble W, Nyenya F, Anderton BH, Hanger DP. Tau phosphorylation affects its axonal transport and degradation. Neurobiology of Aging
Stamer K, Vogel R, Thies E, Mandelkow E, Mandelkow EM. Tau blocks traffic of organelles, neurofilaments, and APP vesicles in neurons and enhances oxidative stress. J Cell Biol
Gandhi S, Abramov AY. Mechanism of oxidative stress in neurodegeneration. Oxid Med Cell Longev
Mohsen MMAE, Iravani MM, SPEcer JPE, Rose S, Fahim AT, Motawi TMK, et al. Age associated changes in protein oxidation and proteasome activities in rat brain: Modulation by anti-oxidants. Biochem Biophys Res Commun
Rajagopal L, Massey BW, Huang M, Oyamada Y, Meltzer HY. The novel object recognition test in rodents in relation to cognitive impairment in schizophrenia. Curr Pharm Des
Kesner RP, Rolls ET. A computational theory of hippocampal function, and tests of the theory: New developments. Neurosci Biobehav Rev
Rolls ET. The mechanisms for pattern completion and pattern separation in the hippocampus. Front Syst Neurosci
Manasathien J, Indrapichate K, Intarapichet K. Antioxidant activity and bioefficacy of Pomegranate Punica granatum
Linn. peel and seed extracts. Global J Pharmacol
Viuda-Martos M, Fernández-Lóaez J, Pérez-álvarez JA. Pomegranate and its many functional components as related to human health: A review. Compr Rev Food Sci Food Saf
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5]