| ORIGINAL ARTICLE |
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| Year : 2019 | Volume
: 12
| Issue : 1 | Page : 14-18 |
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Potentiating activity of rhein in targeting of resistance genes in methicillin-resistant Staphylococcus aureus
Ryong Gong, Dae Young Lee, Jae Won Lee, Doo Jin Choi, Geum-Soog Kim, Sang Hyuk Lee, Young-Seob Lee
Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration, 92 Bisan-ro, Eumsung, Chungbuk, 27709, Korea
Correspondence Address:
Young-Seob Lee
Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration, 92 Bisan-ro, Eumsung, Chungbuk, 27709
Korea
 Source of Support: None, Conflict of Interest: None  | 2 |
DOI: 10.4103/1995-7645.250339
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Objective: To investigate the synergistic effect between rhein (RHE) and oxacillin against Staphylococcus aureus (MRSA) at the gene level. Method: A minimum inhibitory concentration and checkerboard dilution test were conducted to evaluate antibacterial activity. Reverse transcriptase polymerase chain reaction was conducted to investigate the gene expressions. Results: RHE exhibited a minimum inhibitory concentration of 62.5-250.0 μg/mL against various MRSA strains and the reference strain, respectively. As revealed by the checkerboard assay, a combination of RHE and oxacillin exhibited synergistic or partially synergistic effects against MRSA strains. RHE decreased the expressions of mecA/blaZ in a dose-dependent manner. RHE also decreased the expressions of the regulator genes mecI/blaI and mecR1/blaR1. Conclusions: We suggest that RHE affects the activity of mecR1/blaR1, which is located in the cell membrane of MRSA and results in the suppression of mecA/mecI/mecR1 and blaZ/blaI/ blaR1 gene expressions. |
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