| ORIGINAL ARTICLE |
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| Year : 2019 | Volume
: 12
| Issue : 14 | Page : 59-68 |
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Memantine inhibits 6-OHDA-induced apoptosis PC12 cells via the Nurr77 and caspase pathway
Wei Wu1, Rui Wang2, Yong-Mei Fu3, Jie Zhou4, Hai-Chao Huang5, Wei-Wang Gu1
1 Laboratory Animal Center, Southern Medical University, Guangzhou 510515, China
2 Department of Radiology, Peking University Third Hospital, Beijing 100191, China
3 Department of Emergency, the Third Affiliated Hospital of Sun Yat-Sen University, Tianhe Road 600, Guangzhou, Guangdong 510630, China
4 Department of Pharmacy, Sun Yat-Sen University Cancer Center, Dongfengdong Road 651 Guangzhou, Guangdong 510060, China
5 Guangdong Food and Drug Vocational College, Guanzhou 510520, P.R. China
Correspondence Address:
Wei-Wang Gu
Laboratory Animal Center, Southern Medical University, Guangzhou 510515
China
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/1995-7645.271981
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Objective: To investigate the effect of memantine on Parkinson’s disease cell models.
Methods: Parkinson’s disease cell models were established using PC12 cells incubated with 6-hydroxydopamine (6-OHDA). Flow cytometry and microscopy were used to investigate the apoptotic process and the percentage of different apoptotic stages. PC12 cells were infected with lentiviral vectors to knockdown Nur77. Western blotting was used to detect the expression of Nur77 and caspase -3, -8, -9, -12 in PC12 cells withdifferent concentrations of 6-OHDA or 6-OHDA+memantine.
Results: 6-OHDA led to apoptosis PC12 cells, and increased the expression of Nur77 and caspases. Memantine significantly inhibited 6-OHDA-induced apoptosis of PC12 cells. Meanwhile, memantine could mitigate apoptosis of PC12 cells by regulating the Nur77 and caspase pathway.
Conclusions: Memantine has a protective effect on the PC12 cell model via regulating the Nur77 and caspases pathway.
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