| ORIGINAL ARTICLE |
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| Year : 2019 | Volume
: 12
| Issue : 14 | Page : 54-58 |
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Anti-tumor activity of a recombinant endoglin-MIP3α Fc-fusion protein in mice with hepatocellular carcinoma
Zhi-Hui He, Si-Ru Liu, Xin-Lai Wu, Yong-Hao Huang, Yan Chen, Yi Deng, Pan-Pan Xie, Yan-Da Lu
Tumor Institute, the First Affiliated Hospital of Hainan Medical University, Haikou 571199, P.R. China
Correspondence Address:
Yan-Da Lu
Tumor Institute, the First Affiliated Hospital of Hainan Medical University, Haikou 571199
P.R. China
 Source of Support: None, Conflict of Interest: None
DOI: 10.4103/1995-7645.271980
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Objective: To investigate the effects of a recombinant endoglin-macrophage inflammatory protein 3α Fc-fusion protein (EM) vaccine on tumor angiogenesis and growth in mice with H22 hepatocellular carcinoma.
Methods: An in vivo hepatoma mouse model was established. Seven days after subcutaneous inoculation of H22 tumor cells, mice were randomly divided into four groups: EM, endoglin Fc-fusion protein, macrophage inflammatory protein 3α Fc-fusion protein, and normal saline groups. Tumor volume and survival rate of mice were studied at 3-day intervals. Microvessel density of the tumors and tumor cell proliferation were detected by immunohistochemistry, and tumor cell apoptosis was detected by TdT-mediated biotinylated-dUTP nick-end label staining. The number of CD11c and CD86 positive dendritic cells were detected by flow cytometry.
Results: Compared with the other groups, the tumor volume became smaller, and the survival time was longer in the EM-treated group. Besides, microvessel density and cell proliferation index were significantly lower, while the tumor cell apoptosis index was significantly higher in the EM-treated group. Besides the number of CD11c and CD86 positive dendritic cells in EM- treated mice was larger than that in other groups.
Conclusions: EM Fc-fusion protein could effectively inhibit tumor growth through inhibiting endoglin-related tumor angiogenesis and cell proliferation, promoting tumor cell apoptosis, and could induce a certain degree of antitumor immune responses.
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